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Mouse TLR12 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line

Name	Mouse TLR12 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line
Supplier	Novus Biologicals
Catalog	NBP2-26288
Category	Cell Line
Prices	$1,169.00
Sizes	1 vial
Applications	WB FC
Gene	Tlr12
Species	Mouse
Growth Mode	Adherent Morphology: Epithelial
Description	The TLR12 reporter line may be useful for TLR12 based functional studies. The functional activity of the NF-kB/SEAP reporter in the TLR12 stable cell line has been established and validated through PMA activation of the NF-kB pathway. Hence, the cell line may be useful for reseasrchers screening for putative TLR12 ligands activating the NF-kB pathway. TLR12 expression in this cell line has been validated by Western blotting (Fig 1) and flow cytometry (Fig 2). Contents: 3~4 x 10^6 cells Biosafety Level: 2
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Product images

Western Blot: Mouse TLR12 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line [NBP2-26288] - Analysis of TLR12 expression in the TLR12 reporter cell line using an HA antibody (20 ug total protein/lane). Legend. Cont: Control, NF-kB/SEAPorterTM HEK 293 cell line; TLR12: TLR12 reporter cell line.

Flow Cytometry: Mouse TLR12 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line [NBP2-26288] - Expression of TLR12 in TLR12 reporter cell line was analyzed by flow cytometry using a FITC-conjugated TLR12 antibody and compared with the NF-kB/SEAPorter HEK 293 cell line (IML-101). Flow samples were prepared using Intracellular TLR Staining Flow Kit.

Ligand Activation: Mouse TLR12 NF-kB/SEAP - (SEAPorter™) Stable Reporter Cell Line [NBP2-26288] - TLR12 reporter cells were plated in a 96-well plate at 5 x 10^4 cells per well. Cells were stimulated with various concentrations of phorbol 12-myristate 13-acetate (PMA), which is a positive control for NF-kB induction, for 24 h. SEAP was analyzed using the SEAPorter Assay Kit. Data Summary: PMA is a positive control for NF-kB induction. PMA stimulation confirmed that NF-kB/SEAP reporter is functional in Mouse TLR12 SEAP.